Top Abstract Purchase Related
Home
>
Node
>
Subnode

Chemical modification of RNAse from Bacillus intermedius. Effect on the cytotoxic properties of the enzyme

Kurinenko, B.M.; Davydov, R.E.; Bulgakova, R.S.

Antibiotiki i Khimioterapiia 41(7-8): 9-12

1996

ISSN/ISBN: 0235-2990
PMID: 8999766
Document Number: 459036
Modified derivatives of RNAse from Bacillus intermedius differing in the aggregate enzymatic, physico-chemical and physical properties were prepared. The cytotoxic properties of the compounds were tested by the intravital staining of FL cells with neutral red. It was shown that the native enzyme cytotoxicity correlated with the catalytic activity. However, it was found possible to lower the cytotoxicity of the catalytically active compounds by changing the enzyme aggregate properties.

Document emailed within 1 workday
Secure & encrypted payments

Sharipova, F.R.; Filatova, S.V.; Leshchinskaia, I.B. 1988: An intracellular inhibitory activity of RNase secreted by Bacillus intermedius Mikrobiologiia 57(4): 565-570
Bannikova, G.E.; Varlamov, V.P. 1994: Isolation of intracellular inhibitors of bacterial RNAses on a column with immobilized Bacillus intermedius RNAse Prikladnaia Biokhimiia i Mikrobiologiia 30(3): 379-383
Egorov, S.I.; Alimova, F.K.; Zakharova, N.G.; Leshchinskaia, I.B. 1996: Influence of Bacillus intermedius RNAse on growth-stimulating and antagonistic characteristics of Trichoderma harzianum Prikladnaia Biokhimiia i Mikrobiologiia 32(5): 554-556
Gribencha, S.V.; Potselueva, L.A.; Barinskiĭ, I.F.; Balandin, T.G.; Deev, S.M.; Leshchinskaia, I.B. 2004: The antiviral activity of RNAse Bacillus intermedius in experiments with mice preinfected with street rabies virus Voprosy Virusologii 49(6): 38-41
Kendall, L.; Sabbadini, E. 1981: Effect of Bacillus Calmette-Guérin on the in vitro generation of cytotoxic T lymphocytes. I. Effect of BCG on the frequency of cytotoxic T lymphocyte precursors and on the production of helper factors Journal of Immunology 127(1): 234-238
Dobryszycka, W.; Bec-Katnik, I. 1975: Effect of modification on physico-chemical and biological properties of haptoglobin. Acetylation, iodination and nitration Acta Biochimica Polonica 22(2): 143-153
Kharitonova, M.A.; Vershinina, V.I.; Morozova, O.V.; Znamenskaia, L.V. 2006: The role of the promoter structure in the efficiency of the expression of guanylspecific ribonucleases from Bacillus intermedius and Bacillus pumilus Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 15-19
Sharipova, M.R.; Balaban, N.P.; Nekhotiaeva, N.V.; Leshchinskaia, I.B. 2000: The effect of carbon sources and mononucleotides on the production of extracellular alkaline phosphatase by Bacillus intermedius Mikrobiologiia 69(2): 191-196
Bogush, V.G.; Smirnov, V.B.; Rebentish, B.A.; Permogorov, V.I.; Azizbekian, R.R. 1977: Physico-chemical properties of several phages of Bacillus thuringiensis Molekuliarnaia Biologiia 11(4): 901-908
Bondarchuk, A.A.; Vasilevskaya, I.A.; Sergeichuk, M.G. 1982: Physicochemical properties of the elastolytic enzyme complex of Bacillus subtilis Mikrobiolohichnyi Zhurnal 44(4): 8-11
Flemming, C.; Krause, M.; Bock, W.; Gabert, A. 1981: Synthesis and properties of immobilized enzymes. XIII. Modification of the properties of immobilized polygalacturonase following solubilization of an enzyme-dialdehyde sephadex complex Acta Biologica et Medica Germanica 40(7-8): 1067-1069
Büchi, J.; Koller, R.J.; Perlia, X. 1975: Relations between chemical structure, physico-chemical properties, chemical reactivity and effect of various positionally isomeric local anesthetics 31.2 Arzneimittel-Forschung 25(1): 14-24
Visco, C.; Benassi, C.A.; Veronese, F.M.; Miglioli, P.A. 1987: Purification, modification, physico-chemical and pharmacokinetic characterization of arginase, an enzyme of potential use in therapy Il Farmaco; Edizione Scientifica 42(8): 549-559
Weng, G.Y.; Guo, J.L.; Chen, S.Y. 1982: The relationship between physico-chemical properties and enteric dissolution of shellac and its modification Yao Xue Xue Bao 17(5): 395-399
Pikuleva, I.A.; Turko, I.V.; Adamovich, T.B.; Chashchin, V.L. 1990: Chemical modification of rabbit immunoglobulin G by dansylaziridine and study of the properties of modified antibodies Biokhimiia 55(12): 2200-2210
Waugh, D.S.; Pace, N.R. 1993: Gap-scan deletion analysis of Bacillus subtilis RNase P RNA FASEB Journal 7(1): 188-195
Shul'ga, A.A.; Okorokov, A.L.; Panov, K.I.; Kurbanov, F.T.; Chernov, B.K.; Skriabin, K.G.; Kirpichnikov, M.P. 1994: Superproduction of Bacillus intermedius 7P ribonuclease (binase) in Escherichia coli Molekuliarnaia Biologiia 28(2): 453-463
Vinogradov, A.A.; Kudryashova, E.V.; Grinberg, V.Y.; Grinberg, N.V.; Burova, T.V.; Levashov, A.V. 2001: The chemical modification of alpha-chymotrypsin with both hydrophobic and hydrophilic compounds stabilizes the enzyme against denaturation in water-organic media Protein Engineering 14(9): 683-689
Stanĕk, R.; Cinátl, J.; Matousek, J. 1978: The effect of bovine pancreatic ribonuclease (A RNase) and bull seminal ribonuclease (AS RNase) on human lymphocytes undergoing blastic transformation Folia Biologica 24(2): 111-117
Oleĭnikova, G.K.; Shevchenko, L.S.; Kuznetsova, T.A.; Mikhaĭlov, V.V. 1995: A novel fungicide of the iturin group, obtained from a marine isolate of Bacillus subtilis. Isolation, physico-chemical and biochemical properties, identification Antibiotiki i Khimioterapiia 40(2): 19-21