Chemical modification of rabbit immunoglobulin G by dansylaziridine and study of the properties of modified antibodies
Pikuleva, I.A.; Turko, I.V.; Adamovich, T.B.; Chashchin, V.L.
Biokhimiia 55(12): 2200-2210
1990
ISSN/ISBN: 0320-9725 PMID: 2096951 Document Number: 348340
To elucidate the effect of the antigen binding fluorescent thiol reagent, N-dansylaziridine (DAZ) which is sensitive to microenvironmental changes, was used for modification of the rabbit IgG hinge region cystine residue. DAZ binds to the hinge region Cys 226 as could be evidenced from the structural analysis data. Labelling of IgG with DAZ does not alter either its conformation and hydrodynamic behaviour or its antigen binding properties. Upon antigen binding the fluorescence intensity of modified IgG increases up to about 80%. This finding suggests that the interaction of antibodies with the antigen is accompanied by conformational changes in the IgG hinge region.