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Studies on a membrane bound protease from Bacillus pumilus & Escherichia coli

Modi, M.; Wamanacharya, P.; Mehta, B.; Shah, K.; Modi, V.V.

Indian Journal of Experimental Biology 21(11): 611-613

1983

ISSN/ISBN: 0019-5189
PMID: 6373581
Document Number: 206230
The neutral protease activity of particulate fractions from E. coli and B. pumilus decreased gradually with increase in the concentration of penicillin. This enzyme was partially purified and characterized with its MW of .apprx. 48,000. The neutral protease from B. pumilus was inhibited by phenyl methylsulfonyl fluoride (PMSF) and EDTA suggesting that the enzyme required serine group and a divalent cation for its activity. A decline of 57% in Km and 37% in Vmax was observed in the presence of penicillin. The neutral protease from E. coli was inhibited by parachloro mercury benzoate (PCMB) and EDTA, suggesting the involvement of a SH group at the active site and a requirement for a divalent cation. A decline of 84% in Km and 66% in Vmax was observed in the presence of penicillin.

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Eze, M.O.; Okoro, S.I. 1989: Membrane-bound succinate dehydrogenase of Bacillus pumilus strain 5: effects of modulators of monoelectron transfer Microbios 60(242): 45-51
Tseng, C.C. 1981: Studies on pathogenic Escherichia coli. Part 8. Purification and properties of exotoxin from capsular polysaccharide-synthesizing Escherichia coli Journal of the Formosan Medical Association 80(9): 866-872
Kharitonova, M.A.; Vershinina, V.I.; Morozova, O.V.; Znamenskaia, L.V. 2006: The role of the promoter structure in the efficiency of the expression of guanylspecific ribonucleases from Bacillus intermedius and Bacillus pumilus Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 15-19
Tsuji, A.; Torres-Rosado, A.; Arai, T.; Chou, S.H.; Kurachi, K. 1991: Characterization of hepsin, a membrane bound protease Biomedica Biochimica Acta 50(4-6): 791-793
Dzhanga, A.A. 1998: Cloning and sequencing of the chromosome fragment of Bacillus subtilis, complementary to the exoA mutation of Bacillus subtilis and sbcB of Escherichia coli Genetika 34(1): 45-51
Akopian, K.; Zakharian, E.; Kirakosian, G.; Mnatsakanian, N.; Bagramian, K.; Trchunian, A. 2002: Interaction of membrane proton conductivity, membrane and oxidation-reduction potential in Escherichia coli Biofizika 47(6): 1064-1067
Shul'ga, A.A.; Okorokov, A.L.; Panov, K.I.; Kurbanov, F.T.; Chernov, B.K.; Skriabin, K.G.; Kirpichnikov, M.P. 1994: Superproduction of Bacillus intermedius 7P ribonuclease (binase) in Escherichia coli Molekuliarnaia Biologiia 28(2): 453-463
Ferretti, L.; Mottes, M.; Sgaramella, V. 1984: Possible hyperattenuation of transcription in the Escherichia coli hisG gene cloned in Bacillus subtilis Folia Biologica 30(Special Issue): 29-35
Tsoĭ, T.V.; Sakanian, V.A.; Lebedev, A.N.; Alikhanian, S.I. 1981: Cloning and the expression of the DNA promotor fragments of Bacillus thuringiensis in Escherichia coli cells Genetika 17(12): 2100-2104
Sastry, K.S.R.; Tuteja, U.; Bala Krishna, K.; Batra, H.V. 2003: Comparative evaluation of protective antigen produced from Bacillus anthracis and Escherichia coli Indian Journal of Medical Research 117: 111-118
Rabinovich, P.M.; Beburov, M.I.; Linevich, Z.K.; Stepanov, A.I. 1978: Amplification of the riboflavin operon genes of Bacillus subtilis in Escherichia coli cells Genetika 14(10): 1696-1705
Abdulah, R.; Milanda, T.; Sugijanto, M.; Barliana, M.I.; Diantini, A.; Supratman, U.; Subarnas, A. 2017: Antibacterial Properties of Selected Plants Consumed by Primates against Escherichia Coli and Bacillus Subtilis Southeast Asian Journal of Tropical Medicine and Public Health 48(1): 109-116
Sander-Tabaczyńska, A.; Tabaczyński, M.M. 1971: Mutagenic action of edeine in bacteria. II. Induction of streptomycin resistance in Escherichia coli and Bacillus subtilis Acta Microbiologica Polonica. Series A: Microbiologia Generalis 3(1): 29-33
Smirnova, G.V.; Oktiabr'skiĭ, O.N. 1990: Change in the level of SH-compounds in Escherichia coli and Bacillus subtilis cultures during transition to the stationary phase Mikrobiologiia 59(3): 387-393
Yang, R.; Hu, Z.; Deng, Z.; Li, J. 1998: Expression of delta-endotoxin cryIA(c) gene of Bacillus thuringiensis in Escherichia coli and Streptomyces lividans Chinese Journal of Biotechnology 14(2): 59-65
Tsaruk'ianova, I.G.; Osadchaia, A.I. 2007: Joint cultivation of Bacillus subtilis and Escherichia coli strains promising for obtaining complex probiotic Mikrobiolohichnyi Zhurnal 69(2): 43-49
Krupianko, V.I.; Kolot, M.N.; Nesmeianova, M.A. 1981: Comparative study of properties of periplasmic and membrane-bound alkaline phosphatase of E. coli Biokhimiia 46(7): 1249-1257
Jahreis, G.; Aurich, H. 1991: Membrane-bound alanine aminopeptidase from Acinetobacter calcoaceticus. 4. Amino acid chloromethyl ketones as pH-dependent, substrate-derived inhibitors of cysteine protease Biomedica Biochimica Acta 50(9): 1043-1049
Wilhelm, M.; Hollenberg, C.P. 1984: Selective cloning of Bacillus subtilis xylose isomerase and xylulokinase in Escherichia coli genes by IS5-mediated expression EMBO Journal 3(11): 2555-2560
Claeyssen s, M.; Loontiens, F.G.; De Bruyne, C.K. 1978: Determination of the binding parameters of beta-D-xylosidase from Bacillus pumilus, using a fluorescent ligand Archives Internationales de Physiologie et de Biochimie 86(2): 415-416