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Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome

Hinc, K.; Iwanicki, A.; Seror, S.; Obuchowski, Mł.

Acta Biochimica Polonica 53(3): 497-505

2006

ISSN/ISBN: 0001-527X
PMID: 16964327
Document Number: 598389
The genome sequence of the Gram-positive soil bacterium Bacillus subtilis was completed in 1997 (Kunst et al., 1998) and the results included the identification of a putative transcription unit encompassing the yloI to yloS genes. Within this region of the B. subtilis chromosome 11 putative open reading frames were found with a wide diversity of probable functions. In this work we have analyzed transcription in the region of the priA-cpgA genes and we have mapped a promoter which is located inside the priA gene and its activity directs transcription of the def yloM genes. Moreover, this transcript can be extended at low level to the prpC-priK-cpgA genes. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping a A subunit of RNA polymerase. Analysis of the beta-glactosidase activity of transcription fusions revealed that the identified promoter is active at low level and its activity is increased during late exponential phase of growth.

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