Behavior of autonomous plasmids and plasmids integrated into the chromosome during the sporulation of Bacillus subtilis cells
Glumova, E.F.; Prozorov, A.A.
Genetika 22(7): 1099-1103
1986
ISSN/ISBN: 0016-6758 PMID: 3091452 Document Number: 285543
The behaviour of plasmids in free and integrated states was studied upon sporulation of Bacillus subtilis cells. Autonomous plasmids pBD12 and pGG10 were shown to be either transmitted into spores in small copy numbers or completely eliminated from the sporulating cell. However, insertion of the autonomous plasmid into the host chromosome may occur with a certain degree of probability (about 10(-3)) during sporulation. When in the integrated state, pBD12 plasmid may either excise from the host chromosome or amplify within the genome with the probability 1.8-2.10(-3) in the course of sporulation. The pGG102 plasmid carrying the fragment of wheat DNA and integrated by this fragment into the chromosome was shown to enter spores without whichever intragenome rearrangements.