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Construction of recombinant plasmids containing genes of HBsAg and their expression in the eukaryotic cells

Qin, S.; Tang, H.; Zhao, L.; Liu, L.; Lin, Y.

Hua Xi Yi Ke da Xue Xue Bao 32(2): 172-174

2001

ISSN/ISBN: 0257-7712
PMID: 12600076
Document Number: 536587
To construct recombinant plasmids expressing L, M, S and pre-S1-S protein of HBsAg. Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization, identifying the inserting segments by sequencing. The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins. We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.

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Ianishevskiĭ, N.V.; Gintsburg, A.L.; Vertiev, I.V.; Demme, E.I.; Karataev, G.I. 1987: Construction of recombinant plasmids encoding the biosynthesis of the beta-subunit of cholera toxin Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 26-32
Chen, Z.; Li, T.; Hu, S.; Wang, G. 2009: Construction of recombinant adenovirus vector containing rat insulin-like growth factor 1 gene and its expression in islet beta-cells Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 26(5): 1067-1071
Swat, W.; Bułaj, G.; Szopa, J. 1990: Expression of eukaryotic rDNA genes Postepy Biochemii 36(1-2): 4-13
Wu, Z.-J.; Yang, X.-H.; Zheng, S.-S.; Yang, S.-F.; Shi, D. 2004: Construction of human eukaryotic expression plasmid vascular endothelial growth factor 165 and its expression in transfected vascular smooth muscles Hepatobiliary and Pancreatic Diseases International: Hbpd Int 3(3): 355-359
Van Dongen, J.J. 1988: Immunoglobulin genes and T-cell receptor genes and the expression of immunological markers. I. the construction of the specific immune system Nederlands Tijdschrift Voor Geneeskunde 132(19): 855-862
Zhou, N.; Huang, X.-p.; Liao, N.; Wei, S.-l.; Liang, F.-x.; Mai, H.-m. 2007: Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 25(5): 487-489
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Lei, K.; Che, T.-J.; Wang, J.-M.; Deng, N.; Zhang, L.; He, X.-Y. 2009: Mutation analysis of the eda-A1 gene for hypohidrotic ectodermal dysplasia and construction of recombined eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 27(6): 610-613
Schenk, P.M.; Baumann, S.; Mattes, R.; Steinbiss, H.H. 1995: Improved high-level expression system for eukaryotic genes in Escherichia coli using T7 RNA polymerase and rare ArgtRNAs Biotechniques 19(2): 196-198; 200
Ianenko, A.S.; Bekkarevich, A.O.; Akhverdian, V.Z.; Krylov, V.N. 1986: Transfer of chromosomal genes and formation of R'-derivatives using PR4::D312cts15 plasmids in Pseudomonas aeruginosa cells Genetika 22(12): 2784-2793
Han, Q.-q.; Ge, S.-h.; Yan, X.-z.; Yang, P.-s. 2013: Construction and identification of a recombinant lentiviral expression plasmid pLentiTrident1-hBMP2-Neo-hNGF Shanghai Kou Qiang Yi Xue 22(1): 25-29