Top Abstract Purchase Related
Home
>
Node
>
Subnode

Construction and immunological study of recombinant hBD-2/PSMA chimeric protein eukaryotic expressive plasmid

Li, M.; Sun, Y.; Feng, Y.; Wu, Q.; Huang, N.; Wang, B.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 22(2): 283-287

2005

ISSN/ISBN: 1001-5515
PMID: 15884536
Document Number: 587032
The recombinant PSMA DNA vaccine for active immunotherapy of prostate cancer was investigated. Two DNA vaccine recombinant plasmids, pcDNA3.1/PSMA and pcDNA3.1/hBD-2-PSMA, were constructed by inserting the hBD-2 gene and PSMA gene into an eukarytoic expression vector pcDNA3.1. Expression of the two recombinants was detected in transfected COS-7 cells and inoculated mouse muscular cells by RT-PCR and immunohistochemical method. When immunized with pcDNA3.1/PSMA and pcDNA3.1/hBD-2-PSMA, the immunized BALB/c mice acquired specific antibody and T cell response to PSMA. The quantity of the spleen lymphocytes and their CTL activity against PSMA gene transfected-BALB/3T3 cells significantly increased in the immunized mice, and the CTL activity of lymphocytes from pcDNA3.1/hBD-2-PSMA immunized mice was significantly higher than that of pcDNA3.1/PSMA immunized mice. This result suggests that pcDNA3.1/hBD-2-PSMA would probably be developed as a DNA vaccine for the immunotherapy of prostate cancer.

Document emailed within 1 workday
Secure & encrypted payments

Zhang, P.-P.; Li, S.; Yang, P.-S.; Sun, J. 2010: Construction and confirmation of a recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1 Shanghai Kou Qiang Yi Xue 19(6): 635-640
Qin, R.F.; Gu, X.M.; Chen, J.W. 2001: Construction of eukaryotic expression plasmid for mouse myogenic regulatory factor MyoD gene Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 15(5): 257-260
Fu, C.-h.; Tian, L.; Wei, Y.-q.; Wen, Y.-j.; Li, J. 2004: Construction of eukaryotic expression plasmid pSecTag2B-msBlyS expressing mouse soluble B lymphocyte stimulator Hua Xi Kou Qiang Yi Xue Za Zhi 22(2): 145-148
Wu, Z.-J.; Zhang, H.; Yu, L.; Shi, D.; Zheng, S.-S. 2005: Construction of human VEGF165 gene eukaryotic expression plasmid and its effect on proliferation of vascular endothelial cells Hepatobiliary and Pancreatic Diseases International: Hbpd Int 4(3): 364-369
Qin, S.; Tang, H.; Zhao, L.; Liu, L.; Lin, Y. 2001: Construction of recombinant plasmids containing genes of HBsAg and their expression in the eukaryotic cells Hua Xi Yi Ke da Xue Xue Bao 32(2): 172-174
Wang, W-Xia.; Sun, S-Zhen.; Song, Y. 2008: Selection and construction of cell line stably expressing survivin gene in lower level through eukaryotic plasmid vector of shRNA Shanghai Kou Qiang Yi Xue 17(3): 275-279
Grishin, D.V. 2009: Design of a novel chimeric protein construction for hydrolysis of beta-galactosides Antibiotiki i Khimioterapiia 54(5-6): 3-7
Han, Q.-q.; Ge, S.-h.; Yan, X.-z.; Yang, P.-s. 2013: Construction and identification of a recombinant lentiviral expression plasmid pLentiTrident1-hBMP2-Neo-hNGF Shanghai Kou Qiang Yi Xue 22(1): 25-29
Yang, N.-n.; He, K.-f.; Pan, Y.-h.; Wang, H.-n.; Deng, H.; Ma, J.-f. 2012: An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans Shanghai Kou Qiang Yi Xue 21(6): 637-642
Wu, Z.-J.; Yang, X.-H.; Zheng, S.-S.; Yang, S.-F.; Shi, D. 2004: Construction of human eukaryotic expression plasmid vascular endothelial growth factor 165 and its expression in transfected vascular smooth muscles Hepatobiliary and Pancreatic Diseases International: Hbpd Int 3(3): 355-359
Zhou, N.; Huang, X.-p.; Liao, N.; Wei, S.-l.; Liang, F.-x.; Mai, H.-m. 2007: Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 25(5): 487-489
Sveshnikov, P.G.; Solopova, O.N.; Kurbatova, E.A.; Shevchik, I.S.; Baranovskiĭ, P.M.; Ul'ianov, A.M.; Bokov, M.N.; Kiselev, V.I. 2010: Heat-shock protein enhances immunogenicity of E7 oncoprotein of human papillomavirus included in chimeric construction (E7 HPV-HSP70) Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 36-39
Buduwaili, W.-s.; Li, L.; Zhang, B.-c. 2012: Construction of pGL3-hTERT-tk recombinant plasmid and effect of PEG-PEI/Fe(3)0(4) Nano-magnetic fluid-tk/GCV on proliferation of gastric cancer cells BGC823 in vitro Zhonghua Wei Chang Wai Ke Za Zhi 15(7): 727-731
Beliavskaia, V.A.; Timofeev, I.V.; Perminova, N.G.; Paletskaia, T.F.; Kozhina, E.M.; Zagrebel'nyĭ, S.N. 2000: Construction of an expression plasmid vector for accomplishing in vivo delivery of recombinant biologically active proteins. 2. Synthesis of HBcAG in a vaccine strain of Salmonella choleraesuis Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 17-21
Chatton, B.; Bahr, A.; Acker, J.; Kedinger, C. 1995: Eukaryotic GST fusion vector for the study of protein-protein associations in vivo: application to interaction of ATFa with Jun and Fos Biotechniques 18(1): 142-145
Chang, G.T.; Aaldering, L.; Hackeng, T.M.; Reitsma, P.H.; Bertina, R.M.; Bouma, B.N. 1994: Construction and characterization of thrombin-resistant variants of recombinant human protein S Thrombosis and Haemostasis 72(5): 693-697
Sorokin, V.M.; Goncharov, E.K.; Mishan'kin, B.N. 1989: Transcription map of a recombinant plasmid carrying a gene for the synthesis of pesticine i and a protein for pesticine i immunity in the plague microbe Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 6: 39-42
Ogasawara, M. 1986: A study on the veto cells from H-2 recombinant mice and allogeneic bone marrow chimeric mice Hokkaido Journal of Medical Science 61(6): 883-891
Coelho, Vânia.; Dernedde, J.; Petrausch, U.; Panjideh, H.; Fuchs, H.; Menzel, C.; Dübel, S.; Keilholz, U.; Thiel, E.; Deckert, P.Markus. 2007: Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy in colon cancer International Journal of Oncology 31(4): 951-957
Xue, J.; Peng, J.; Zhang, L.; Liu, S.; Chen, J.; Wang, A.; Yuan, M.; Xu, W.; Lu, S. 2010: Construction of recombinant adenovirus vector pAdxsi-green fluorescent protein-homo sapiens NEL-like 1 and transfected into rat bone marrow mesenchymal stem cells Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 24(5): 606-612