Top Abstract Purchase Related
Home
>
Node
>
Subnode

Transcription map of a recombinant plasmid carrying a gene for the synthesis of pesticine i and a protein for pesticine i immunity in the plague microbe

Sorokin, V.M.; Goncharov, E.K.; Mishan'kin, B.N.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 6: 39-42

1989

ISSN/ISBN: 0208-0613
PMID: 2811901
Document Number: 340347
Molecules of the plasmids pBR322 and pRD17 have been compared by electron microscopy technique of R-loops visualization. Comparative location of R-loops on the plasmids has been computerized on minicomputer HP9825A due to the program making possible to define the coordinates of the transcription start and direction. The 5.1 kb fragment coding for pesticin I and immunity protein to pesticin I and cloned in pBR322 vector plasmid is flanked by Bam HI-EcoRI sites. Five promoter regions and direction of transcription were localized on the fragment.

Document emailed within 1 workday
Secure & encrypted payments

Klausa, V.I.; Nivinskas, R.G. 1988: Determination of the direction of the transcription of bacteriophage T4 genes by heat induction of the transcription from recombinant plasmid Pl-promoter: 2 directions in the region of genes 25-29 Genetika 24(1): 42-52
Ryzhko, I.V.; Shcherbaniuk, A.I.; Moldavan, I.A.; Tsuraeva, R.I.; Anisimov, B.I.; Trishina, A.V. 2007: Efficacy of cefixime and cefepime vs. other cephalosporins in experimental plague of albino mice due to variants FI+ and FI- of the plague microbe Antibiotiki i Khimioterapiia 52(9-10): 24-27
Byvalov, A.A.; Pautov, V.N.; Chicherin, I.V.; Lebedinskiĭ, V.A.; Evtigneev, V.I. 1984: Effectiveness of revaccinating hamadryas baboons with NISS live dried plague vaccine and fraction i of the plague microbe Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 74-76
Goncharov, A.I.; Goncharov, E.K.; Alutin, I.M.; Marchenkov, V.I. 1992: Localization of the DNA segment coding for the synthesis of fraction i on the pYT plague pathogen plasmid Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1992(11-12): 10-14
Rudas, V.A.; Piven', N.M.; Rivkin, M.I.; Vershinin, A.V.; Infante, D.C. 1997: The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene Tsitologiia i Genetika 31(2): 17-22
Stougaard, P.; Light, J.; Molin, S. 1982: Convergent transcription interferes with expression of the copy number control gene, copA, from plasmid R1 EMBO Journal 1(3): 323-328
Krashennikova, L.V.; Fedotov, A.R.; Tarasov, V.A. 1986: Mutants of ColE1 plasmid with altered expression of the colicin E1 immunity gene Genetika 22(6): 914-919
Dunaev, G.S.; Zykin, L.F.; Prozorovskiĭ, S.V.; Kostiukovskiĭ, V.M.; Kurilov, V.I. 1978: Production of L forms of the plague microbe Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 10: 85-89
Li, M.; Sun, Y.; Feng, Y.; Wu, Q.; Huang, N.; Wang, B. 2005: Construction and immunological study of recombinant hBD-2/PSMA chimeric protein eukaryotic expressive plasmid Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 22(2): 283-287
Balakhonov, S.V.; Voronova, G.A.; Shestopalov, M.I.; Bazanova, L.P.; Tokmakova, E.G. 2000: The use of the polymerase chain reaction for the detection of the plague microbe in fleas Meditsinskaia Parazitologiia i Parazitarnye Bolezni 3: 29-31
Gorbaseva, T.I.; Makarovskaia, L.N.; Miliutin, V.N.; Kirdeev, V.K. 1980: Action of ampicillin on the plague microbe (Y. pestis EV) according to electron microscopic data Antibiotiki 25(7): 503-507
Fomenko, D.E.; Dziumenko, E.V.; Khmel', I.A. 1997: Participation of the rpoS gene in expression of plasmid genes determining synthesis of C51 microcin Russian Journal of Genetics 33(2): 223-225
Bozhko, N.V.; Lebedeva, S.A.; Bichul', O.K.; Ivanova, V.S.; Lysova, L.K.; Barabash, G.P.; Nekliaev, V.N.; trukhachev, A.L.; Terent'ev, A.N. 2005: Primary characterization of the properties and diagnostic value of the antigenic complex "fraction V" in a plague microbe Klinicheskaia Laboratornaia Diagnostika 6: 45-49
Lesovoĭ, V.S.; Berezina, O.N.; Manolov, A.D.; Luk'ianenko, I.I. 1984: Detection of a specific plague microbe antigen (fraction I) by the method of radioimmunological analysis Laboratornoe Delo 3: 185-186
Shimaniuk, N.I.; Mishan'kin, B.N. 1982: Effect of the cultivation temperature on the enzymes of sialic acid metabolism in the plague microbe Mikrobiolohichnyi Zhurnal 44(6): 49-53
Ryzhko, I.V.; Shcherbaniuk, A.I.; Samokhodkina, E.D.; Tsuraeva, R.I.; Mishn'kin, B.N.; Kasatkina, I.V.; Zhigalova, T.A. 1994: Virulence of rifampicin and quinolone resistant mutants of strains of plague microbe with Fra+ and Fra- phenotypes Antibiotiki i Khimioterapiia 39(4): 32-36
Yang, N.-n.; He, K.-f.; Pan, Y.-h.; Wang, H.-n.; Deng, H.; Ma, J.-f. 2012: An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans Shanghai Kou Qiang Yi Xue 21(6): 637-642
Beliavskaia, V.A.; Timofeev, I.V.; Perminova, N.G.; Paletskaia, T.F.; Kozhina, E.M.; Zagrebel'nyĭ, S.N. 2000: Construction of an expression plasmid vector for accomplishing in vivo delivery of recombinant biologically active proteins. 2. Synthesis of HBcAG in a vaccine strain of Salmonella choleraesuis Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 17-21
Avanian, I.A.; Sosnikhina, T.M. 1972: The biochemical characteristics of the erythrocytes of guinea pigs with hypocatalasemia and the sensitivity of these animals to the plague microbe Voprosy Meditsinskoi Khimii 18(4): 371-376
Gao, B.-a.; Xiong, W.-n.; Xu, Y.-j.; Zhang, Z.-x.; Cao, Y.; Tang, Y.-j.; Ye, T.; Du, C.-l. 2006: Effects of recombinant adeno-virus vector carrying interleukin-5 antisense on the expression of interleukin-5 mRNA and protein in CD4+ T lymphocytes of asthmatic rats Zhonghua Nei Ke Za Zhi 45(4): 298-301