Top Abstract Purchase Related
Home
>
Node
>
Subnode

Effects of recombinant adeno-virus vector carrying interleukin-5 antisense on the expression of interleukin-5 mRNA and protein in CD4+ T lymphocytes of asthmatic rats

Gao, B.-a.; Xiong, W.-n.; Xu, Y.-j.; Zhang, Z.-x.; Cao, Y.; Tang, Y.-j.; Ye, T.; Du, C.-l.

Zhonghua Nei Ke Za Zhi 45(4): 298-301

2006

ISSN/ISBN: 0578-1426
PMID: 16780678
Document Number: 597043
To construct recombinant adeno-associated virus vector carrying antisense interleukin-5 (IL-5) gene (rAAV-asIL-5), and to explore the effects of this virus transfection on IL-5 mRNA and protein in CD(4)(+) T lymphocytes of asthmatic rats. The eukaryotic antisense IL-5 expressing vector plasmid of recombinant adeno-associated virus (pasIL-5/rAAV) was constructed by gene recombination technique. The rAAV-asIL-5 particles were produced by co-transfection of pasIL-5/rAAV, pXX2, and pXX6 in package cell 293 through phosphate calcium deposit, and the titers of rAAV-asIL-5 were measured by Southern blot. The rAAV-asIL-5 particles were transfected into CD(4)(+) T lymphocytes obtained by gradient of Ficoll and immunomagnetic beads from the peripheral blood of asthmatic rats. Then IL-5 mRNA in T lymphocytes and IL-5 protein in supernatant of cell culture were determined with semi-quantitative RT-PCR and ELISA respectively. (1) The rAAV-asIL-5 was constructed and identified, and the titer of rAAV-asIL-5 was 1.3 x 10(11) virus particles/ml. (2) The relative ratio A of absorbance (IL-5/beta-actin) of rAAV group was 1.0515 +/- 0.1477, which was significantly lower than that of the control group (1.4271 +/- 0.1655) (n = 6, P < 0.01). (3) The protein level of IL-5 in supernatant of culture of rAAV group was (12.0840 +/- 1.4769) ng/L, significantly lower than that of the control group [(15.3590 +/- 1.2685) ng/L, n = 6, P < 0.01]. Construction of rAAV-asIL-5 was successful, and transfection of this virus was capable of inhibiting the expression of IL-5 mRNA and protein in CD(4)(+) T lymphocytes of asthmatic rats. The results of this study provide experimental data for further study of gene therapy for asthma.

Document emailed within 1 workday
Secure & encrypted payments

Weinstein, J.A.; Taylor, J.M. 1987: Interleukin-1 and the acute-phase response: induction of mouse liver serum amyloid a mRNA by murine recombinant interleukin-1 Journal of Trauma 27(11): 1227-1232
Crawford, D.H.; Thomas, J.A.; Gregory, C.D.; Catovsky, D.; Chaggar, K. 1995: Induction of latent membrane protein expression in in vitro Epstein-Barr virus-infected leukaemic B lymphocytes by interleukin 4 and antibodies to CD40 Leukemia 9(5): 747-753
Aebersold, D.M.; Banholzer, R.; Backenstoss, V.; Hirsch, H.H.; Moroni, C. 1997: V-Ha-ras-dependent expression of interleukin-3 mRNA in premalignant PB-3c mast cells correlates with the formation of tumors without interleukin-3 gene rearrangements Experimental Hematology 25(5): 395-404
Takii, T.; Hayashi, H.; Marunouchi, T.; Onozaki, K. 1994: Interleukin-1 down-regulates type I interleukin 1 receptor mRNA expression in a human fibroblast cell line TIG-1 in the absence of prostaglandin E2 synthesis Lymphokine and Cytokine Research 13(3): 213-219
Hakim, A.A. 1988: Peripheral blood lymphocytes from patients with cancer lack interleukin-2 (IL-2) receptor expression: isolation of mRNA encoding IL-2R Cancer Detection and Prevention 12(1-6): 59-71
Sun, A.; Chu, C.T.; Liu, B.Y.; Wang, J.T.; Leu, J.S.; Chiang, C.P. 2000: Expression of interleukin-2 receptor by activated peripheral blood lymphocytes upregulated by the plasma level of interleukin-2 in patients with recurrent aphthous ulcers Proceedings of the National Science Council Republic of China. Part B Life Sciences 24(3): 116-122
Shi, Z.; Fan, L.; Qiang, H.; Tang, Y.; Wang, K.; Dang, X. 2010: Study on biological activity of recombinant adeno-associated virus vector co-expressing human vascular endothelial growth factor 165 and human bone morphogenetic protein 7 genes in vitro Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 24(4): 415-419
Kierszenbaum, F.; Lopez, H.M.; Sztein, M.B. 1996: Trypanosoma cruzi downregulates the production of interleukin-2, interferon-gamma, interleukin-4 and interleukin-5 by activated human lymphocytes Journal of Parasitology 82(4): 652-656
Kan, H.; Song, W.; Jiang, R. 2001: Effect of airborne fungi on interleukin-4 mRNA expression in the peripheral blood mononuclear cells of rats Wei Sheng Yan Jiu 30(5): 269-270
Li, H.-H.; Zhang, S.-M.; Fang, S.-Y.; Chen, C.-L.; Luo, Y.-D.; Guan, Y.; Wang, D.-W.; Xiao, X. 2003: Adeno-associated virus vector carrying human minidystrophin gene SMCKA3999 effectively ameliorates dystrophic pathology in mdx model mice Zhonghua Yi Xue Za Zhi 83(17): 1513-1516
Koppold, B.; Sauer, G.; Büning, H.; Hallek, M.; Kreienberg, R.; Deissler, H.; Kurzeder, C. 2005: Efficient gene transfer of CD40 ligand into ovarian carcinoma cells with a recombinant adeno-associated virus vector International Journal of Oncology 26(1): 95-101
Chen, Z.; Colon, I.; Ortiz, N.; Callister, M.; Dong, G.; Pegram, M.Y.; Arosarena, O.; Strome, S.; Nicholson, J.C.; Van Waes, C. 1998: Effects of interleukin-1alpha, interleukin-1 receptor antagonist, and neutralizing antibody on proinflammatory cytokine expression by human squamous cell carcinoma lines Cancer Research 58(16): 3668-3676
Qiu, H.B.; Chen, D.C.; Pan, J.Q.; Liu, D.W.; Ma, S. 1999: Inhibitory effects of nitric oxide and interleukin-10 on production of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6 in mouse alveolar macrophages Zhongguo Yao Li Xue Bao 20(3): 271-275
Zhou, X.; Wang, Z.; Lin, Y.; Zhu, Y. 2001: The effects of adenosine, interleukin-1 and theophylline on the expression of A2 adenosine receptor mRNAs in peripheral blood mononuclear cells from asthmatic patients Hua Xi Yi Ke da Xue Xue Bao 32(2): 204-207
Sims, J.E.; Smith, D.E. 2003: Regulation of interleukin-1 activity is enhanced by cooperation between the interleukin-1 receptor type Ii and interleukin-1 receptor accessory protein European Cytokine Network 14(2): 77-81
Itoh, K.; Shiiba, K.; Ebina, N.; Anzai, R.; Ouchi, A.; Matsuno, S. 1993: Functional and phenotypic analysis of tumor infiltrating lymphocytes derived from solid tumors and effusion associated lymphocytes in malignant ascites cultured in recombinant interleukin 2 Nihon Geka Gakkai Zasshi 94(8): 781-790
Puri, R.K.; Travis, W.D.; Rosenberg, S.A. 1989: Decrease in interleukin 2-induced vascular leakage in the lungs of mice by administration of recombinant interleukin 1 alpha in vivo Cancer Research 49(4): 969-976
Airoldi, I.; Guglielmino, R.; Carra, G.; Corcione, A.; Gerosa, F.; Taborelli, G.; Trinchieri, G.; Pistoia, V. 2002: The interleukin-12 and interleukin-12 receptor system in normal and transformed human B lymphocytes Haematologica 87(4): 434-442
Wang, G.; Yu, C.-J.; Dong, X.-Y.; Tian, W.-H.; Wu, X.-B. 2012: Recombinant adeno-associated virus type 8 mediated dual-luciferase gene expression in mouse Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 26(6): 439-441
Gnatenko, D.; Arnold, T.E.; Zolotukhin, S.; Nuovo, G.J.; Muzyczka, N.; Bahou, W.F. 1997: Characterization of recombinant adeno-associated virus-2 as a vehicle for gene delivery and expression into vascular cells Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research 45(2): 87-98