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The induction of auxotrophic mutations for riboflavin by the integration of plasmid pLRS33 into the chromosome of Bacillus subtilis

Shevchenko, T.N.; Timashova, E.O.; Gorinchuk, G.F.; Maliuta, S.S.

Genetika 24(8): 1371-1374

1988

ISSN/ISBN: 0016-6758
PMID: 3144477
Document Number: 318430
The transformation of Bacillus subtilis Lys- strains with plasmid pLRS33 containing pBR322 and the Bac. subtilis chromosomal fragment carrying the genes for lysin biosynthesis and the riboflavin operon regulatory operator region (ribO) leads to the appearance of Rib- mutants. It was shown that these mutants contained long deletions covering a great portion of the riboflavin operon.

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