Optimization of conditions for immobilization of alpha-amylase from Bacillus sp. BKL20 in Ca2+-alginate beads
Kubrak, O.I.; Lushchak, V.I.
Ukrains'kyi Biokhimichnyi Zhurnal 80(6): 32-41
2008
ISSN/ISBN: 0372-3909 PMID: 19351055 Document Number: 620071
The method of alpha-amylase from Bacillus sp. immobilization by an entrapment in Ca2+-alginate beads has been performed in the paper. Maximum content of immobilized alpha-amylase was observed at neutral pH values during immobilization. The approximately equal quantities of alpha-amylase were entrapped into Ca2+-alginate beads, that was confirmed by the similar total activity (approximately 0.05 U) and declining specific activity (from 12 to 1 U/mg protein) of immobilized alpha-amylase after the addition of different amounts of enzyme preparation (from 20 to 160 mg of protein) to 1 ml of 2% alginate solution. Alginate concentration affected the activity of immobilized enzyme, maximum alpha-amylase activity was observed at low concentrations of the carrier (0.15-0.25%), used to prepare beads. alpha-Amylase, entrapped in Ca2+-alginate beads, was successfully tested during five hydrolysis cycles.