Optimization of culture conditions of Aspergillus niger for the synthesis of alpha-N-acetylgalactosaminidase and alpha-galactosidase
Borzova, N.V.; Malanchuk, V.M.; Varbanets, L.D.; Seĭfullina, I.I.; Zubkov, S.V.
Mikrobiolohichnyi Zhurnal 63(4): 27-36
2001
ISSN/ISBN: 0201-8462 PMID: 11692674 Document Number: 536015
Different factors have been investigated for their effect on the process of biosynthesis of alpha-N-acetylgalactosaminidase and alpha-galactosidase of Aspergillus niger under deep-water cultivation. Optimal sources and concentrations of carbon (soy flour--25 g/l) and nitrogen (pepton--7.5 g/l) were estimated. It has been established that temperature of 25 degrees C, pH 6.0, growing in 50 ml medium at the swing velocity 220 rev/min. for 6-8 days are optimal cultivation parameters. It has been shown that dry borine blood (in concentration of 1%) and a number of guanidine derivatives (guanidine carbonate--0.25%, nitroaminoguanison dimethylaminobenzaldehyde--0.05%, nitroaminoguanison of salicylic aldehyde--0.1%) can play the part of inducers of the mentioned glycosidase synthesis. When growing fungal culture in selected conditions synthesis of enzymes increased almost 3 times. Activity of alpha-N-acetylgalactosaminidase was 0.46 E/ml, and alpha-galactosidase--1.9 E/ml.