Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
Krasevec, N.; van den Hondel, C.A.; Komel, R.
Pflugers Archiv European Journal of Physiology 439(3 Suppl): R84-R86
2000
ISSN/ISBN: 0031-6768 PMID: 10653151 Document Number: 524008
A gene-fusion expression strategy was applied for the heterologous expression of hTNF-alpha in A. niger AB1.13. The TNF-alpha gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not present in the culture medium. From the expression of two hTNF-alpha analogues, LK 811 (Cys95/148) and LK 802 (Cys95/148, His107/108) respectively, we concluded that oligomerisation was not the critical point for secretion of hTNF-alpha in A. niger, but more probably improper folding already at the stage of monomer formation, or even incorrect processing of the molecule during the secretion pathway.