N-acetyl-beta-D-hexosaminidase secreted by the marine fungus Phoma glomerata
Zhuravleva, N.V.; Luk'ianov, P.A.; Pivkin, M.V.
Prikladnaia Biokhimiia i Mikrobiologiia 40(5): 520-526
2004
ISSN/ISBN: 0555-1099 PMID: 15553782 Document Number: 569001
Among the ten strains of marine fungi studied, the mycelial fungus Phoma glomerata showed maximum potency in producing N-acetyl-beta-D-glucosaminidase. The conditions for fungal growth and enzyme biosynthesis were evaluated. N-Acetyl-beta-D-hexosaminidase was isolated from the culture liquid of Phoma glomerata by ion-exchange chromatography (on DEAE-cellulose and DEAE-Sephacell) and gel filtration (on Toyopearl HW-55) with a yield of 35%; the enzyme, purified 36.4-fold, had a molecular weight of 20 kDa. The homogeneity of the enzyme was confirmed by gel filtration and SDS-PAGE. Transglycosylation reactions catalyzed by the enzyme produced N-acetyl-D-glucosamine and N-acetyl-D-galactosamine with respective yields of 38 and 46%.