Optimization of immobilization conditions for acid proteinase from Aspergillus awamori
Motina, L.I.; Samoshina, N.M.; Erin, A.E.; Kestner, A.I.; Nakhapetian, L.A.
Prikladnaia Biokhimiia i Mikrobiologiia 14(3): 411-413
1978
ISSN/ISBN: 0555-1099 PMID: 674118 Document Number: 128849
To optimize the immobilization conditions for acid proteinase from Aspergillus awamori by covalent binding through glutaraldehyde, experiments were carried out using the Box-Wilson method. The optimization process was assessed on the basis of absolute activity A, coefficient of activity retention gamma and their product A gamma. The following conditions can be recommended: glutaraldehyde concentration 50--60 mg/g, enzyme concentration not less than 40 mg/g, time of glutaraldehyde treatment 2--2.5 hrs, immobilization time 2 hrs, pH about 4.0, and temperature 35--40 degrees C. Under these conditions A=220--230 U/g, gamma = 23--24% Agamma = 5,000--6,000.