Study of the photochemical immobilization of alkaline proteinase and chymotrypsin on the solid phase of O-hydroxyethylcellulose
Sroková, I.; Cík, G.
Zentralblatt für Mikrobiologie 148(4): 247-252
1993
ISSN/ISBN: 0232-4393 PMID: 8368024 Document Number: 411947
Stability of the photochemically immobilized alkaline proteinase (E.C. 3.4.21.14) and chymotrypsin (E.C. 3.4.21.1.) onto the gel of O-hydroxyethylcellulose has been studied. For the purpose of immobilization the photochemical generation of nitrene radicals caused by the photolysis of an azido group of bifunctional 4,4'-bis-azidostilbene-2,2'-disodium-sulphate and the newly synthetized O-(3-azidophthaloyl)-O-hydroxyethylcellulose have been employed. The immobilized alkaline proteinase demonstrated a decreased ability of denaturation and an increased laboratory stability.