Concentration and purification of alkaline proteinase by ultrafiltration
Shishkova, E.A.; Fokina, S.S.; Trefilov, E.M.; Fedorenko, B.N.; Gorenkova, I.M.
Prikladnaia Biokhimiia i Mikrobiologiia 17(2): 233-237
1981
ISSN/ISBN: 0555-1099 PMID: 6787579 Document Number: 172448
The mode of ultrafiltration of the culture fluid of Bacillus subtilis, producer of alkaline proteinase, through semipermeable acetate cellulose membranes was examined. The ultrafiltration of the enzyme-containing solutions involved both concentration of the solution and enzyme purification from low molecular weight admixtures. The optimum mode of ultrafiltration of alkaline proteinase on UAM membranes "Vladipore" was developed.