Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography
Labriola, C.; Sousa, M.; Cazzulo, J.J.
Biological Research 26(1-2): 101-107
1993
ISSN/ISBN: 0716-9760 PMID: 7670520 Document Number: 422117
The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity.