Purification of Meerrettich-peroxidase by means of affinity chromatography on concanavalin A-agarose
Wagner, M.
Acta Biologica et Medica Germanica 34(8): 1429-1431
1975
ISSN/ISBN: 0001-5318 PMID: 1211002 Document Number: 92363
Crude preparations of horse-radish peroxidase were purified by means of affinity chromatography on Concanavalin A-agarose. The peroxydase was bound to Concanavalin A, whereas the majority of other proteins of the preparation pass through the column. Subsequently the peroxidase was eluted by means of 1 M sucrose with high purity. The purified enzyme is convenient for the immunoenzyme technique.
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