Affinity chromatography of acetylcholinesterase from Electrophorus electricus electroplax. Investigations on 9-aminoalkylacridine affinity ligands

Taylor, J.L.; Allmond, W.K.; Himel, C.M.

Journal of Chromatography 257(2): 275-284

1983


ISSN/ISBN: 0021-9673
PMID: 6853624
Document Number: 204372
The neural enzyme 11-S acetylcholinesterase (E.C. 3.1.1.7) was purified by affinity chromatography from a trypsin digest of E. electricus electric organ. Unquaternized affinity ligands were reported which were comparable in efficacy to the routinely employed quaternized acridine MAC 10-methylacridinium ligand. A study was made of the quaternization reactions of various 9-aminoalkylacridines and 9-aminoacridine along with their relative binding affinities to acetylcholinesterase. Ease of synthesis in conjuction with the column performance of these unquaternized 9-aminoalkylacridine compounds made them the preferred affinity ligand in acetylcholinesterase chromatography. A new carbodiimide synthetic route for these unquaternized ligands was described.

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