Isolation of cathepsins D by affinity chromatography
Kazakova, O.V.; Orekhovich, V.N.
Biokhimiia 40(5): 969-972
1975
ISSN/ISBN: 0320-9725 PMID: 1212453 Document Number: 94796
The paper deals with the isolation of cathepsin D from rat liver, chicken liver and bovine spleen by affinity chromatography. The synthesis of the adsorbent was performed using the competitive inhibitor of cathepsins D and pepsin pepstatin and activated Sepharose. Application of a pepstatin-Sepharose column allows obtaining a highly active and purified enzyme in a short period of time.
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