An approach to isolating individual tRNA from the human placenta by affinity chromatography. Isolation of highly purified Phe-tRNA Phe
Zarytova, V.F.; Karpova, G.G.; Mundus, D.A.; Shishkina, I.G.
Bioorganicheskaia Khimiia 16(8): 1069-1072
1990
ISSN/ISBN: 0132-3423 PMID: 2285425 Document Number: 360541
A new approach to isolation of individual tRNAs from eukaryotes based on affinity chromatography is suggested. At first, using a sorbent with oligonucleotide pTGGT attached, the total tRNA with native CCA-ends was obtained. Then by means of a sorbent with oligonucleotide pTTCAG immobilized, which is complementary to a part of the tRNA(Phe) anticodon loop, tRNA(Phe) with the acceptor activity greater than 1000 pmole/unit was isolated.