Biological activity of tRNA, aminoacyl-tRNA-synthetases and composition of their high molecular weight complexes in regenerating rat liver
Iaremchuk, A.D.; El'skaia, A.V.
Ukrainskii Biokhimicheskii Zhurnal 55(4): 363-367
1983
ISSN/ISBN: 0201-8470 PMID: 6623663 Document Number: 216819
tRNA isolated from the liver of rats with partial hepatectomy is established to possess an increased acceptor activity for amino acid mixture, lysine and glutamic acid 21 h after the operation and a decreased activity for tryptophan 6 and 15 h later when comparing control and experimental animals. The content of endogenic aminoacyl-tRNA in the liver of control and experimental animals in all periods of regeneration corresponds to the maximum level of tRNA aminoacylation in vitro. A complete correlation of changes in the tRNA activity and aminoacyl-tRNA-synthetases is observed only for glutamyl-tRNA-synthetase and tRNAGlu. High-molecular complexes of ARSases (codosomes) are isolated from liver of control and experimental animals 21 h after partial hepatectomy. The activity of lysyl- and glutamyl-tRNA-synthetases, content of tRNA, tRNAGlu,Lys as compared to the control is determined to rise, the content of phospholipids in their composition being 24% higher.