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Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27

Iaremchuk, A.D.; Tukalo, M.A.; Egorova, S.P.; Konovalenko, A.V.; Matsuka, G.K.

Ukrainskii Biokhimicheskii Zhurnal 62(2): 97-99

1990

ISSN/ISBN: 0201-8470
PMID: 2368192
Document Number: 351965
A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.

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