Production of polyclonal antibodies to tyrosyl-tRNA-synthetase from the bovine liver and characterization of two forms of the enzyme
Rozhko, O.T.; Korneliuk, A.I.; Ribkhinska, T.A.; Savinskaia, L.N.; Odynets, K.A.; Matsuka, G.K.
Ukrainskii Biokhimicheskii Zhurnal 69(3): 9-16
1997
ISSN/ISBN: 0201-8470 PMID: 9505355 Document Number: 482404
The polyclonal antibodies purified by affine chromatography against tyrosyl-tRNA synthetase (TyrRS) immobilized on the column with affigel-sepharose have been obtained from the bovine liver. The immunospecificity of these antibodies and their influence on enzymatic activity of TyrRS from the bovine liver have been investigated. We have stated that the polyclonal antibodies inhibited TyrRS enzymatic activity in aminoacylation of homologous tRNA(Tyr) by 47%. As it has been shown by immunoblotting the antibodies reacted both with the native enzyme (M(r) 2.59 kDa) and with the proteolytic cleaved functionally active form of the enzyme (M 2.39 kDa).