Enzyme immunoassay for the determination of antibodies against bovine leukosis virus
Meyer, U.; Schilow, W.F.
Archiv für Experimentelle Veterinarmedizin 38(2): 219-222
1984
ISSN/ISBN: 0003-9055 PMID: 6331334 Document Number: 241016
This quantitative indirect immunoenzyme technique uses rabbit antiserum to bovine IgG conjugated to alkaline phosphatase, and bovine oncovirus antigen adsorbed to PVC slips. Of six serum samples from cattle, five were positive. The weakest positive reaction was confirmed by the leukocyte adherence inhibition test and by the syncytium test, but the immunodiffusion test was negative. Further work is needed to increase the specificity of the test.
Document emailed within 1 workday
Related Documents
Rothe, U.; Lasch, J.; Römer, I.; Sandow, D.; Hübner, G.; Rosmus, K.; Kiessig, S.T.; Porstmann, T. 1986: Determination of antibodies to rubella virus with the disperse dye immunoassay (DIA) in comparison with an enzyme-linked immunosorbent assay (ELISA) Biomedica Biochimica Acta 45(10): 1325-1332Maris, P.; Girre, L. 1984: Purification of the 2 major antigens of bovine leukosis virus for the purpose of immunoenzymatic detection of antibodies to this virus Annales Pharmaceutiques Francaises 42(1): 57-67
Miliukiené, V.; Dikiniené, N.; Vidziūnaité, R.; Mikalauskiené, G.; Veleckaité, A.; Mikulskis, P. 1991: The comparative evaluation of spectrophotometric and chemiluminescent detection in an immunoenzyme test of antibodies to the antigens of the bovine leukosis virus Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 7: 64-66
Straub, O.C. 1983: Effect of vaccination against foot-and-mouth disease on results of the immunodiffusion test for the demonstration of precipitating bovine leukosis virus antibodies Deutsche Tierärztliche Wochenschrift 90(4): 135-137
Pohl, W.D.; Tischner, H. 1984: Comparative studies on the detection of antibodies to influenza virus antigen using enzyme immunoassay and counterimmunoelectrophoresis Zeitschrift für Erkrankungen der Atmungsorgane 163(2): 187-190
Minning, P.; Hlinak, A.; Klaczinski, K. 1993: Development of an enzyme immunoassay for the detection of antibodies from hens and mice against Newcastle disease virus Berliner und Munchener Tierarztliche Wochenschrift 106(5): 149-153
Burny, A.; Bex, F.; Chantrenne, H.; Cleuter, Y.; Dekegel, D.; Ghysdael, J.; Kettmann, R.; Leclercq, M.; Leunen, J.; Mammerickx, M.; Portatelle, D. 1978: Bovine leukemia virus involvement in enzootic bovine leukosis Advances in Cancer Research 28: 251-311
Herring, A.J.; Nettleton, P.F.; Burrells, C. 1980: A micro-enzyme-linked immunosorbent assay for the detection of antibodies to infectious bovine rhinotracheitis virus Veterinary Record 107(7): 155-156
Cardosa, M.J.; Hah, F.L.; Choo, B.H.; Padmanathan, S. 1993: Screening of pig sera for antibodies to Japanese encephalitis virus using a dot enzyme immunoassay and IgM capture ELISA: comparison with the hemagglutination inhibition and plaque reduction neutralization tests Southeast Asian Journal of Tropical Medicine and Public Health 24(3): 472-476
Genov, I.; Tsutsumanski, V. 1986: Role of saliva in transmission of bovine leukosis virus Archiv für Experimentelle Veterinarmedizin 40(3): 387-388
Yoshikawa, H.; Yoshikawa, T.; Oyamada, T.; Sasaki, T.; Koyama, H.; Tsubaki, S. 1992: Establishment and maintenance of bovine leukosis virus-free farm Kitasato Archives of Experimental Medicine 65(2-3): 137-141
Paunesku, G.; Manolesku, N. 1986: Correlations between enzootic bovine leukosis virus and cellular immune system Archiv für Experimentelle Veterinarmedizin 40(3): 336-338
Nötzel, U.; Jantschak, J.; Nyakatura, G.; Prösch, S.; Beyer, W.; Drescher, B.; Rosenthal, S.; Rosenthal, H.A. 1987: Cloning and characterization of recombinants of bovine leukosis virus (BLV) from BLV-infected fetal lamb kidney cells Archiv für Experimentelle Veterinarmedizin 41(5): 697-702
Nishimura, Y.; Yamaguchi, K.; Takatsuki, K.; Kiyokawa, T.; Imamura, Y.; Fujiwara, H. 1989: Evaluation of the confirmatory test by western blotting assay (WB) for the detection of antibodies to adult T-cell leukemia virus type I. Analysis of the discrepancy between indirect immunofluorescence assay (IF), enzyme immunoassay (EIA) and gelatin particle agglutination assay (PA) Rinsho Byori. Japanese Journal of Clinical Pathology 37(4): 385-389
Courault, K.; Quast, M. 1985: Detection of anti-IgA antibodies using enzyme immunoassay Folia Haematologica 112(6): 928-936
Hildreth, S.W.; Beaty, B.J. 1984: Detection of eastern equine encephalomyelitis virus and Highlands J virus antigens within mosquito pools by enzyme immunoassay (EIA). I. A laboratory study American Journal of Tropical Medicine and Hygiene 33(5): 965-972
Teppo, A.M. 1981: Enzyme immunoassay of antibodies to Sjögren's syndrome B antigen Clinical Chemistry 27(8): 1341-1345
Miyawaki, S. 1994: Clinical evaluation of quantitative measurement of various antinuclear antibodies by enzyme immunoassay Rinsho Byori. Japanese Journal of Clinical Pathology 42(5): 467-475
Bradley, L.A.; Franco, E.L.; Reisner, H.M. 1984: Use of monoclonal antibodies in an enzyme immunoassay for factor VIII-related antigen Clinical Chemistry 30(1): 87-92
Song, A.-j.; Li, X.-h.; Li, J.; Xu, S.-h.; Zhang, F.; Wan, Y.-m.; Zhang, C.-t.; Wang, Y.-c. 2003: Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 17(4): 319-321