Determination of antibodies to rubella virus with the disperse dye immunoassay (DIA) in comparison with an enzyme-linked immunosorbent assay (ELISA)
Rothe, U.; Lasch, J.; Römer, I.; Sandow, D.; Hübner, G.; Rosmus, K.; Kiessig, S.T.; Porstmann, T.
Biomedica Biochimica Acta 45(10): 1325-1332
1986
ISSN/ISBN: 0232-766X PMID: 3566722 Document Number: 282045
A disperse dye immunoassay (DIA) using Fantagen-dye sol particles as label was developed for the measurement of rubella antibodies in human serum specimen. The DIA has a sensitivity comparable to the hemagglutination-inhibition (HI) test, whereas the detection limit for a similar ELISA-system developed in parallel is lower by a factor of about two orders of magnitude. The results of testing 34 random serum specimens with the 3 different methods showed a significant correlation (v = 0.73 for DIA vs. ELISA). Titer rises in 10 paired sera (acute/convalescent) estimated with the DIA- and ELISA-methods were in good agreement with H1-results. Only two of the 10 pairs differed in titer rise by no more than one dilution step.