Detection of anti-neutrophil antibodies by enzyme linked immunosorbent assay--comparison of results obtained by ELISA with those by leukocyte agglutination test and granulocyte cytotoxicity test
Hayashi, S.; Kiyokawa, T.; Aochi, H.; Nagamine, K.; Oshida, M.; Tomiyama, Y.; Kurata, Y.
Rinsho Byori. Japanese Journal of Clinical Pathology 42(5): 527-533
1994
ISSN/ISBN: 0047-1860 PMID: 8022088 Document Number: 428280
We examined anti-neutrophil antibodies in 12 patients with various disorders using enzyme-linked immunosorbent assay (ELISA), and the results obtained by ELISA were compared with those obtained by leukocyte agglutination test (LAT) and granulocyte cytotoxicity test (GCT). IgG anti-neutrophil antibody was positive in 7 of 12 patients, and IgM type antibody was positive in 6 patients. There was a significant correlation between IgG and IgM anti-neutrophil antibodies. The results obtained by ELISA were not in accord with those obtained by LAT or GCT. In addition, we examined anti-neutrophil antibodies in serially collected serum samples from two patients with immune neutropenia. The results obtained by ELISA correlated with their neutrophil counts, suggesting that anti-neutrophil antibodies detected by ELISA have pathological relevance.