Alkylation of tRNA-Phe free and bound to phenylalanyl-tRNA synthetase with 4- (N-2-chloroethyl-N-methylamino) benzylamine
Vlasov, V.V.; Grishaev, M.P.; Mamaev, S.M.; Chizhikov, V.E.; Khodyreva, S.N.
Molekuliarnaia Biologiia 15(1): 45-53
1981
ISSN/ISBN: 0026-8984 PMID: 7038443 Document Number: 176142
Reactivities toward the alkylating reagent 4-(N-2-chloroethyl-N-methylamino)benzylamine of guanosine residues in the tRNAPhe free and bound to the phenylalanyl-tRNA synthetase were determined. This highly efficient reagent modifies mainly guanosine in single-stranded as well as in helical regions of tRNAs. The reaction is sensitive to the elements of the macrostructure of tRNAs. It is found the phenylalanyl-tRNA synthetase protects the D-stem of the tRNAPhe (guanosines G10, G22, G24) from alkylation.