The use of DNA polymerase from Thermus thermophilus
Kuz'min, A.N.; Kuz'min, V.G.; Morozov, V.G.
Voprosy Virusologii 37(2): 94-95
1992
ISSN/ISBN: 0507-4088 PMID: 1441435 Document Number: 403587
National DNA polymerase from Thermus thermophilus was used in polymerase chain reaction (PCR). The synthetic oligonucleotides (primers) to the basic structural HIV genes GAG, ENV, the genome DNA of donor peripheral blood lymphocytes were used, and the controls included the plasmid DNA with cloned HIV genome and the genome DNA of peripheral blood lymphocytes from HIV-infected persons confirmed by ELISA and Western blot analysis. The PCR technique and evaluation of the obtained results are described. The expediency of using PCR for different contingents is discussed.