Isolation of alpha2-macroglobulin from human plasma using combination of pseudoligand affinity chromatography and gel filtration
Smorodin, E.P.; Arukaévu, K.E.
Voprosy Meditsinskoi Khimii 35(5): 83-86
1989
ISSN/ISBN: 0042-8809 PMID: 2482578 Document Number: 330917
Combination of two procedures--pseudoligand affinity chromatography on Cibacron blue agarose and usual gel filtration on TSK HW-60 enabled to separate successfully blood plasma alpha 2-macroglobulin from immunoglobulin M, haptoglobins and low molecular weight proteins. The preparation of alpha 2-macroglobulin obtained exhibited high inhibitory activity towards proteases.
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