Elimination of cholesterol by affinity chromatography
Berezin, I.V.; Lopukhin, I.M.; Andrianov, I.P.; Lapuk, I.I.; Alekseeva, L.B.
Voprosy Meditsinskoi Khimii 26(6): 843-846
1980
ISSN/ISBN: 0042-8809 PMID: 7456416 Document Number: 156020
Elimination of cholesterol from physiological fluids was carried out by means of affinity chromatography using digitonine as an affinity agent. For this purpose a method of covalent binding of digitonine with aminosylochrome was developed. The most suitable conditions for production of the stable sorbent specific for cholesterol are described. The ratio of concentrations of digitonine to sodium periodate was 1:2; oxidation was performed in a medium containing 50% aqueous dioxane within 1.5 hrs. The modified sorbent, used for affinity chromatography of cholesterol, possessed a specific capacity of 10 mg/g.