Purification of a special estrogen-binding protein from the liver of rats by affinity chromatography on estradiol sepharose

Smirnov, A.N.; Shchelkunova, T.A.; Egorova, V.V.; Reshetova, I.G.; Chernoburova, E.I.; Smirnov, A.N; Shchelkunova, T.A; Egorova, V.V; Reshetova, I.G; Chernoburova, E.I

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Purification of a special estrogen-binding protein from the liver of rats by affinity chromatography on estradiol sepharose

Smirnov, A.N.; Shchelkunova, T.A.; Egorova, V.V.; Reshetova, I.G.; Chernoburova, E.I.

Biulleten' Eksperimental'noi Biologii i Meditsiny 98(9): 377-380

1984

ISSN/ISBN: 0365-9615

PMID: 6487802

Document Number: 227487

A procedure has been developed for purification of a special rat liver estrogen-binding protein. It includes protein precipitation by ammonium sulfate, gel filtration, ion exchange chromatography, and affinity chromatography on estradiol sepharose. The protein is purified 2260-fold with a 27% yield. Upon electrophoresis in 10% PAG in the presence of sodium dodecylsulfate the protein gives one polypeptide strip with a molecular weight of 31.200 +/- 400 dalton.