Immobilized IDA-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase
Yang, L.; Jia, L.; Zou, H.; Kong, L.; Zhang, Y.
Se Pu 15(4): 292-295
1997
ISSN/ISBN: 1000-8713 PMID: 15739458 Document Number: 479807
Cu2+-chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined to purify bovine liver catalase (BLC) by affinity chromatography. The buffer pH notably affect BLC binding to the Cu2+-chelating membrane adsorbent. The results showed that under the chromatographic conditions chosen, 67.7% recovery of BLC attained with an overall 4.7-fold increase in specific activity in a single step. After purification of BLC, the chelating membrane adsorbent can easily be regenerated by imidazole or EDTA buffer. Different results were obtained by the two regeneration methods: the later is more effective than the former in reviving the purification characters of the chelating membrane adsorbent.