Purification of an alkaline phosphatase-lipoprotein-X complex
Weijers, R.N.; Kruijswijk, H.; Baak, J.M.
Clinical Chemistry 26(5): 604-608
1980
ISSN/ISBN: 0009-9147 PMID: 7261305 Document Number: 165214
An alkaline phosphatase isoenzyme was observed in an abnormal position in the agar-agarose gel pattern of sera from several patients suffering from intrahepatic and extrahepatic cholestasis. We purified this isoenzyme, by gel filtration and affinity chromatography, from the serum of a patient suffering from extrahepatic cholestasis. Analysis demonstrated an alkaline phosphatase-lipoprotein-X complex with a relative molecular mass of at least 669,000. We discuss the interpretation of alkaline phosphatase isoenzyme patterns produced by different techniques.