The ultrafiltration at pre-analytical stage under detection of concentration of lactic acid in blood plasma
Alekseevskaia, E.S.; Zhloba, A.A.; Subbotina, T.F.
Klinicheskaia Laboratornaia Diagnostika 11: 27-30
2013
ISSN/ISBN: 0869-2084 PMID: 24640105 Document Number: 667004
The detection of concentration of lactic acid in blood plasma and other objects is especially applied to discover the mitochondria dysfunctions. The study was organized to analyze samplings of blood plasma and plasma ultra-filtrates taken from 80 healthy persons and 73 patients with activation of intravascular coagulation and fibrinolysis using lactate-oxidase test. The comparative analysis of results of detection of concentrations of lactic acid in blood plasma and its ultra-filtrate established that in 72% of cases the higher values of concentration of detecting lactic acid took place after procedure of ultra-filtration enabling separation of overwhelming quantity of protein. In accordance with accumulated experience in the field of clinical diagnostic practice the enzyme tests are to be applied to detect the concentration of lactic acid in blood plasma and other objects. The present study demonstrated the expediency of application of plasma ultra-filtrate to detect the concentration of lactic acid.