Optimization of protein hydrolysis by Bacillus subtilis alkaline proteinase
Bogatkov, S.V.; Frolova, T.T.; Gracheva, I.M.; Nekliudov, A.D.; Loginova, T.A.
Prikladnaia Biokhimiia i Mikrobiologiia 18(1): 71-75
1982
ISSN/ISBN: 0555-1099 PMID: 6806800 Document Number: 191102
Casein hydrolysis by alkaline proteinase from Bacillus subtilis str. 72 had been investigated. It has been shown that the hydrolytic rate depends on the substrate concentration and declines rapidly with time even at low protein concentrations. It has been suggested to use an equation describing the time dependence of the rate of casein proteolysis and characterizing the optimum time of protein hydrolysis, maximum rate of hydrolysis, and the maximum yield of the reaction products.