Metabolic-intermediate complex formation reveals major changes in rat hepatic cytochrome P-450 subpopulations in addition to those forms previously purified after phenobarbital, beta-naphthoflavone, and isosafrole induction
Bornheim, L.M.; Franklin, M.R.
Molecular Pharmacology 21(2): 527-532
1982
ISSN/ISBN: 0026-895X PMID: 7099151 Document Number: 191103
By coupling a simple separatory procedure with the ability of cytochrome P-450 subpopulations to sequester themselves as metabolic-intermediate (MI) complexes, previously undocumented alterations of cytochrome P-450 subpopulations by inducing agents were detected. Phenobarbital induced at least 2 forms of cytochrome P-450 differing in their chromatographic properties and MW, with neither form possessing the ability to generate MI complexes from isosafrole. .beta.-Naphthoflavone induced at least 2 forms having different chromatographic properties, MW and abilities to generate MI complexes; these 2 forms differed from both of the forms induced by phenobarbital. Isosafrole induced at least 3 forms. All of generated MI complexes but differed from each other in chromatographic properties and MW. In addition to the well-characterized forms of cytochrome P-450, phenobarbital, .beta.-naphthoflavone and isosafrole also caused changes in uncharacterized forms and it was these other forms which were responsible for the changes in isosafrole MI complex formation seen after .beta.-naphthoflavone and phenobarbital treatment.