Additional induction of microsomal cytochrome (s) P 450 in mouse liver by consecutive application of cadmium chloride and phenobarbital
Dieter, H.H.; Abel, J.; Ohnesorge, F.K.
Research Communications in Chemical Pathology and Pharmacology 24(2): 377-388
1979
ISSN/ISBN: 0034-5164 PMID: 461991 Document Number: 144065
Mice were treated intraperitoneally over 20 days with 0,30; 0,10 and 0,03 mg Cd+2/kg.day. Subsequent induction of microsomal metabolism by 50 mg phenobarbital/kg.day over 5 days revealed increased microsomal cytochrome P 450 content in Cd+2-pretreated animals as compared to animals treated only with phenobarbital. The microsomal cadmium content of animals having received cadmium plus phenobarbital was about 5 times higher than that of animals treated with cadmium alone. The only enzyme activity found to be changed by cadmium in induced microsomes was ethylmorphine-N-deethylation. In phenobarbital-free animals no microsomal changes by cadmium could be detected.