Immunological study of the identity of cytochrome P-448 in the mouse and rat liver after induction with 3-methylcholanthrene
Mishin, V.M.; Guliaeva, L.F.; Mishina, D.V.; Gutkina, N.I.
Voprosy Meditsinskoi Khimii 30(5): 98-102
1984
ISSN/ISBN: 0042-8809 PMID: 6442038 Document Number: 237853
As shown by means of Ouchterlony double immunodiffusion technique after induction with 3-methyl cholanthrene the antibodies towards cytochrome P-448 from rat liver tissue developed a clear-cut precipitation line with homologous antigen and interacted also with cytochrome P-448 from mice liver tissue although the precipitation was less distinct. Antibodies towards mice liver cytochrome P-448 reacted also with the rat liver cytochrome P-448 but less distinctly as compared with the mice cytochrome. At the same time, these antibodies inhibited similarly the benz(a)-pyrene-hydroxylase reaction, catalyzed by both mice and rat cytochromes P-448. Partial immunological identity appears to occur in the cytochromes studied. Role of total antigenic determinants in enzymatic reactions catalyzed by cytochrome P-448 is discussed.