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Preparation of heteroenzyme conjugates: trypsin-chymotrypsin and trypsin-alkaline phosphatase

Rajput, Y.S.; Gupta, M.N.

Biotechnology and Applied Biochemistry 10(3): 242-250

1988

ISSN/ISBN: 0885-4513
PMID: 2840096
Document Number: 323018
Hybrid enzymes which have two different enzyme activities linked together covalently may be useful reagents for various applications, such as the determination of complex biological structures. The present paper describes the preparation and purification of two such enzyme-enzyme conjugates, namely, trypsin-chymotrypsin and trypsin-alkaline phosphatase. Whereas the former has been prepared by using the well-known bifunctional reagent glutaraldehyde, the latter exploited the Schiff base formation between the oxidized carbohydrate moiety of alkaline phosphatase and the free amino groups of trypsin.

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