The effects of trypsin, plasmin, chymotrypsin and thrombin on human heart adenylate cyclase activity
Cros, G.H.; Chaptal, P.A.; Serrano, J.J.
Research Communications in Chemical Pathology and Pharmacology 57(2): 277-280
1987
ISSN/ISBN: 0034-5164 PMID: 2958913 Document Number: 292791
The authors report the effects of four proteases (trypsin, plasmin, chymotrypsin and thrombin) on human heart adenylate cyclase (HHAC) activity. Trypsin and plasmin inhibited HHAC at concentrations higher than 0.3 and 1 microgram/ml, respectively. Chymotrypsin had a biphasic effect, with a stimulation (from 0.5 to 10 micrograms/ml) followed by an inhibition at higher concentrations. Maximal stimulation was obtained at 3 micrograms/ml and averaged 67.2 +/- 5.4%. Thrombin had no significant effect at concentrations up to 1 mg/ml. These data indicate that proteases might regulate HHAC and therefore influence cardiac function.