Comparative study on activity of adenylate cyclase in normal human ovary with ovarian tumors
Qiu, M.; Chen, M.; Du, Z.
Hua Xi Yi Ke Da Xue Xue Bao 23(2): 168-170
1992
ISSN/ISBN: 0257-7712 PMID: 1452150 Document Number: 390510
It has been documented that the binding activity sites of human chorionic gonadotropin (HCG) receptor and kd in human ovarian tumors are different from those in the normal ovary. And some observations suggest that the HCG receptor depends on adenylate cyclase (AC) for its physiological functions. We studied the activity of AC in normal human ovary and ovarian tumors. Five human ovarian specimens and eighteen ovarian tumor specimens were obtained from women patients undergoing gynecological surgery. Ovaries were homogenized and sonicated. The homogenates were centrifuged at 1000 .times. g for 15 min. After sucrose density gradient ultracentrifugation (78,000 .times. g, 4 h), the membrane fraction was collected from interface between 33% and 37%. The membrane protein approximately 10 .mu.g, ATP 1 mmol/L, 3H-ATP 5 .times. 104 cpm, sulphydrylethyl alcohol 10 mmol/L, in a final volume of 200 .mu.l of Tris-HCl buffer (50 mmol/L), pH 7.5, containing MgSO4 5 mmol/L, were incubated at 35.degree. C for 10 min. The reaction was stopped by boiling water bath for 3 min. The AC activity (U/mg): of human normal ovary, 67 .+-. 8, of cystadenocarcinoma serous, 146 .+-. 70; of cystadenocarcinoma mucinous, 289 .+-. 83.