The effect of proteases on the synthesis of glucoamylase by mutants of Aspergillus niger C
Fiedurek, J.; Paszczyński, A.; Ilczuk, Z.
Acta Microbiologica Polonica 34(1): 25-32
1985
ISSN/ISBN: 0137-1320 PMID: 2579524 Document Number: 245608
Out of 1230 cultures grown from conidia of the strain Aspergillus niger C. which were previously treated with mutagens, 6 mutants were selected, 5 of which were characterized by considerably lower or lack of proteolytic activity than the parent strain. It was observed that glucoamyulase activity and protein synthesis in the mutants examined were directly proportional to the rate of their proteolytic activity. From electrophoretic analysis it was found that in some protease-free mutants or with strongly reduced activity of this enzyme one or two glucoamylase fractions out of four occurring in the parent strain were absent.