Preparation of an active strain of Bacillus licheniformis--producer of thermostable alpha-amylase
Tsurikova, N.V.; Nefedova, L.I.; Kostyleva, E.V.; Zvenigorodskiĭ, V.I.; Iasinovskiĭ, V.G.; Voeĭkova, T.A.; Sinitsyn, A.P.
Prikladnaia Biokhimiia i Mikrobiologiia 38(5): 502-508
2002
ISSN/ISBN: 0555-1099 PMID: 12391749 Document Number: 543789
A highly potent strain of Bacillus licheniformis 103 that synthesized thermostable alpha-amylase with temperature and pH optima of 90-95 degrees C and 6.0-8.5, respectively, was obtained by mutagenesis and selection. The composition of fermentation media and conditions for submerged cultivation of the producer were optimized. alpha-Amylase whose activity reached 260 U/ml was obtained in laboratory fermenters.