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A new Enzyme Preparation with High Penicillopepsin Activity Based on the Producer Strain Penicillium canescens

Smirnova, I.A.; Sereda, A.S.; Kostyleva, E.V.; Tsurikova, N.V.; Bushina, E.V.; Rozhkova, A.M.; Sinitsyn, A.P.

Prikladnaia Biokhimiia i Mikrobiologiia 51(6): 584-591

2015

ISSN/ISBN: 0555-1099
PMID: 26859960
Document Number: 683049
The producer of fungal penicillopepsin, an aspartate protease, has been created by genetic engineering. The biochemical and physicochemical properties of the penicillopepsin enzyme preparation obtained from the culture liquid of the producer were studied. Properties of the new enzyme preparation and the commercially available aspergillopepsin were compared. Their proteolytic activities were found to be 670-680 U/g of the preparation. The soluble protein yield upon the wheat flour hydrolysis with penicillopepsin was 2.7 times higher than with aspergillopepsin. It is probably caused by the presence of the xylanase activity in the penicillopepsin preparation.

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