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The antigenic properties of hepatitis A virus particles possessing different physicochemical characteristics

Kusov, I.Iu.; Nastashenko, T.A.; Kazachkov, I.A.; Donets, M.A.; Grishina, G.K.; Berkova, N.P.; Balaian, M.S.

Voprosy Virusologii 36(3): 203-206

1991

ISSN/ISBN: 0507-4088
PMID: 1654006
Document Number: 369178
A modified enzyme immunoassay based on adsorption of antihepatitis A virus (HAV) IgG-HRPO conjugate and monoclonal antibodies to HAV were used to investigate antigenic differences between mature HAV virions and subviral particles with different buoyant densities in CsCl produced in HAV-infected cells. The mature virions (1.34 g/cm3) appeared to have common antigenic determinants with subviral particles (1.20, 1.27, and 1.30 g/cm3) and possess some additional determinants. Nevertheless, both subviral particles and mature virions induced antibodies capable of neutralizing HAV infectivity in tissue culture.

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