Physicochemical and biological characteristics of preparations of an antigenic complex isolated from the cultivation medium of Bordetella pertussis
Semina, I.E.; Bazhanova, I.G.; Tsvetkova, N.V.; Remova, T.N.; Ozeretskovskaia, M.N.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 8: 76-79
1988
ISSN/ISBN: 0372-9311 PMID: 2904201 Document Number: 325111
The physicochemical and biological properties of antigenic complexes isolated from the supernatant fluid of the culture medium of B. pertussis, strains 305 and 475, were studied. The preparations obtained from both strains contained proteins, carbohydrates, nucleic acids and lipids. Electrophoresis in polyacrylamide gel revealed the presence of filamentous hemagglutinin, 4 subunits of B. pertussis toxin and agglutinogens in the antigenic complexes of both strains. The preparations of both strains possessed similar toxic properties and, after their detoxification, produced a pronounced protective effect.