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A comparative study of monoclonal and monospecific antibodies in determining the immunodominant antigenic sites of influenza virus a (H3N2) hemagglutinin

Isaeva, E.I.; Rovnova, Z.I.; Podcherniaeva, R.I.

Voprosy Virusologii 36(1): 7-10

1991

ISSN/ISBN: 0507-4088
PMID: 1858361
Document Number: 380487
Comparative studies of monospecific (MSA) and monoclonal (MCA) antibodies showed MSA to detect three non-overlapping immunodominant sites on the surface of hemagglutinin (HA) molecule whereas MCA established more subtle differences in HA antigenic structure on the level of epitopes with different immunological significance. The activity of MSA and MCA differed in various tests. While MCA were more active in HI and EIA tests, MSA had a higher neutralizing activity, reducing the infectious virus titre by 5.0-7.5 Ig. Similar reduction of the virus biologic activity was observed only with two MCA whereas the other 20 MCA had a poor neutralizing effect (the virus titre reduction not more than by 2.5 Ig). The employment of MSA and MCA gives most complete information on antigenic restructuring of influenza virus HA at the site level and more subtle structures, epitopes, in the process of evolutionary variability.

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