Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC
Li, X.-d.; Ma, Z.-j.; Lin, S.; Gu, X.-z.; Mao, S.-j.
Zhongguo Zhong Yao Za Zhi 29(6): 537-539
2004
ISSN/ISBN: 1001-5302 PMID: 15706918 Document Number: 570958
To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae. Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm. Synephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%. This method can be used for quality control of Citri Reticulatae.